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Publications

Journal Article Epublish

PubMed: 40790296 DOI: 10.1038/s41377-025-01931-w

Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE).

Light Sci Appl 14(1):266

Authors: Daixuan Wu, Yuecheng Shen, Zhongzheng Zhu, Tijian Li, Jiawei Luo, Zhengyang Wang, Jiaming Liang, Zhiling Zhang, Yunhua Yao, Dalong Qi, Lianzhong Deng, Zhenrong Sun, Meng Liu, Zhi-Chao Luo, Shian Zhang

Affiliations

  • Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong, 510006, China.
  • State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai, 200241, China.
  • State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai, 200241, China.
  • State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai, 200241, China. School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China.
  • Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong, 510006, China. MOE Key Laboratory of Laser Life Science, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
  • State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai, 200241, China. Joint Research Center of Light Manipulation Science and Photonic Integrated Chip of East China Normal University and Shandong Normal University, East China Normal University, Shanghai, 200241, China. Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, 030006, China.

Abstract

Single-pixel imaging (SPI) is a promising technology for optical imaging beyond the visible spectrum, where commercial cameras are expensive or unavailable. However, limitations such as slow pattern projection rates and time-consuming reconstruction algorithms hinder its throughput for real-time imaging. Consequently, conventional SPI is inadequate for high-speed, high-resolution tasks. To address these challenges, we developed an ultrahigh-throughput single-pixel complex-field microscopy (SPCM) system utilizing frequency-comb acousto-optic coherent encoding (FACE). This system enables real-time complex-field monitoring in the non-visible domain. Operating at 1030 nm, our system achieves a record-high space-bandwidth-time product (SBP-T) of 1.3 × 10 7, surpassing previous SPCM (~10 4), SPI (~10 5), and even certain types of commercial near-infrared cameras (~10 6). It supports real-time streaming at 1000 Hz with a frame size of 80 × 81 pixels and a lateral resolution of 3.76 μm across an approximately 300 μm field of view. We validated the system by imaging dynamic transparent scenes, including microfluidics, live microorganisms, chemical reactions, as well as imaging through scattering media. This advancement offers a superior solution for high-speed, high-resolution complex-field imaging beyond the visible spectrum, significantly enhancing SPI performance across various applications.

MeSH

Solutions, Microscopy