Journal Article Epublish
PubMed: 40804422 DOI: 10.1186/s40001-025-02951-3
The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats.
Eur J Med Res 30(1):749
Authors: Samah Fouad, Marwa Abass, Awad Rizk, Esam Mosbah, Mostafa M Nabeeh, Ayman S Elmezayyen, M I El-Henawey, Adel Zaghloul
Affiliations
- Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt.
- Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
- Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
- Department of Neurosurgery, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt.
- Physics Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt.
- Physics Department, Faculty of Science, New Mansoura University, New Mansoura, 35516, Egypt.
Abstract
BACKGROUND
Epidural fibrosis (EF) is the major complication that develops in the operative region of the spinal vertebrae. This fibrous scar connects the connective tissue around the lateral nerve roots and epidural, resulting in severe pain post-spinal operation and impairment of the nerves' function.
PURPOSE
This study was conducted to investigate the effect of using platelet-rich fibrin (PRF), chitosan, and their combination in reducing epidural fibrosis after laminectomy in rats.
METHODS
Ninety male Sprague Dawley rats weighing 255 ± 55 g were randomly assigned to five groups, each consisting of 15 rats: the normal group (control), the laminectomy group, the PRF group, the chitosan group, the combination group (PRF/chitosan), and the donor group. All rats, except the control group, had lumbar laminectomy surgeries between L3 and L5. Macroscopic analysis, histological evaluations, and mRNA analysis for TGFβ-1 and IL6 were compared statistically after a 30-day follow-up.
RESULTS
In comparison to the laminectomy group, the EF area was significantly decreased in the PRF, chitosan, and combination groups. Histological study, macroscopic inspection, and mRNA expression of TGFβ-1 (P < 0.0001) and IL6 (P < 0.0001) show that the use of PRF with chitosan topically in dura following laminectomy resulted in a decrease in scar tissue formation, inflammation, and EF post-laminectomy.
CONCLUSION
The combination of chitosan with PRF is a potential therapeutic approach for minimizing EF in rats after laminectomy.
MeSH
Fibrosis, Cicatrix, Clinical Medicine, Laminectomy, Chitosan, Blood Platelets, Tissues, Platelet-Rich Fibrin, Fibrin, Spine