Journal Article Epublish
PubMed: 41002622 DOI: 10.3390/jcdd12090343
Non-Invasive Mapping of Ventricular Action Potential Reconstructed from Contactless Magnetocardiographic Recordings in Intact and Conscious Guinea Pigs.
J Cardiovasc Dev Dis 12(9)
Authors: Riccardo Fenici, Marco Picerni, Peter Fenici, Donatella Brisinda
Affiliations
- School of Medicine and Surgery, Catholic University of the Sacred Heart, 00168 Rome, Italy.
- Biomagnetism and Clinical Physiology International Center, 00144 Rome, Italy.
- Mathematics Area, SISSA-International School for Advanced Studies, 34136 Trieste, Italy.
- General Medicine and Aging Unit, Fondazione Policlinico Universitario A. Gemelli, Istituto di Ricerca e Cura a Carattere Scientifico (IRCCS), 00168 Rome, Italy.
Abstract
Optical mapping, nanotechnology-based multielectrode arrays and automated patch-clamp allow transmembrane voltage mapping with high spatial resolution, as well as L-type calcium and inward rectifier currents measurements using native mammalian cardiomyocytes. However, these methods are limited to in vitro and ex vivo experiments, while magnetocardiography (MCG) might offer a novel approach for non-invasive preclinical safety assessments of new drugs in intact and even conscious rodents by reconstructing the ventricular action potential waveform (rVAPw) from MCG signals. Objective: This study aims to assess the feasibility of rVAPw reconstruction from MCG signals in Guinea pigs (GPs) and validate the results by comparison with simultaneously recorded epicardial ventricular monophasic action potentials (eVMAP). Methods: Unshielded MCG (uMCG) data of 18 GPs, investigated anaesthetized and awake at ages of 5, 14, and 26 months using a 36-channel DC-SQUID system, were analyzed to calculate rVAPw from MCG's current arrow map. Results: Successful rVAPw reconstruction from averaged MCG showed good alignment with eVMAP waveforms. However, some rVAPw displayed incomplete or distorted repolarization at sites with lower MCG amplitude. Conclusions: 300-s uMCG averaging allowed rVAPw reconstruction in intact GPs. Occasionally distorted rVAPw suggests the need for dedicated MCG devices development, with higher density of optimized vector sensors, and modelling tailored for small animal hearts.
MeSH
Action Potentials, Magnetocardiography