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Publications

English Abstract Journal Article Epublish

PubMed: 41147578 DOI: 10.36233/0507-4088-294

[Genetic diversity of human metapneumovirus (Pneumoviridae: Metapneumovirus) in Russia: results of molecular analysis].

Vopr Virusol 70(2):164-176

Authors: A V Fadeev, Y V Ivanov, P A Petrova, A A Perederiy, M M Pisareva, A D Moshkin, A B Komissarov, D M Danilenko, D A Lioznov

Affiliations

  • A.A. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation.
  • Research Institute of Virology, Federal Research Center for Fundamental and Translational Medicine.

Abstract

INTRODUCTION

Human metapneumovirus (hMPV) holds significant epidemiological importance, being a dominant cause of lower respiratory tract infections in children under two years of age and individuals over 65. Multiple infections with hMPV throughout a person's life are possible due to the antigenic and genetic variability of the virus. However, the genetic variability of hMPV circulating in Russia remains unexplored.

AIM OF THE STUDY

The aim of this study was to test a protocol for whole-genome sequencing of hMPV to assess the genetic diversity of metapneumoviruses circulating in certain regions of Russia.

MATERIALS AND METHODS

Nasopharyngeal swabs were collected from patients of different ages with acute respiratory viral infections (ARVI) tested positive for hMPV using polymerase chain reaction (PCR). From some of the samples, viral isolates were obtained in cell culture. Whole-genome sequencing was performed on both swabs and isolates using the MiSeq Illumina platform, followed by phylogenetic analysis.

RESULTS

For the first time in Russia, whole-genome sequencing of 44 hMPV strains circulating from 2017 to 2024 was conducted. Their genetic group affiliation was described, with the A2b2 clade shown to dominate. It was confirmed that the greatest variability among genes encoding viral surface proteins was observed in the G gene, while changes in the F gene were minimal during the studied period.

CONCLUSION

The study provides insights into the genetic diversity of hMPV strains circulating in various regions of the Russian Federation. Understanding the genetic variability of hMPV is crucial for comprehending viral evolution, transmission dynamics, and mechanisms of immune evasion, which influence the development of vaccines and antiviral drugs.

MeSH

Genetic Variation, Genes, Polymerase Chain Reaction, Genome, Metapneumovirus, Viruses, Antiviral Agents, Infections, Virus Diseases