Journal Article Ppublish
PubMed: 41501980 DOI: 10.1111/jmp.70056
Characterization of Hypoxia-Preconditioned Umbilical Cord Mesenchymal Stem Cells Derived From Macaca fascicularis.
J Med Primatol 55(1):e70056
Authors: Ratih Rinendyaputri, Frans Dany, Sela Septima Mariya, Novaria Sari Dewi Panjaitan, Uly Alfi Nikmah, Hieronimus Adiyoga Nareswara, Alvian Dumingan, Sonny Sonny, Rio Handi Sanjaya, Amarila Malik, Rahardianty Hanum Tazkia, Yulia Irnidiyanti, Hasta Handayani Idrus, Wireni Ayuningtyas, Rachmawati Noverina, Fathul Huda, Ahmad Faried
Affiliations
- Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Bogor, Indonesia.
- Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
- Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia.
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia.
- Bio Farma Stem Cell Research and Development, Bandung, Indonesia.
- Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, RS UNPAD University Hospital, West Java, Indonesia.
- Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran - RS UNPAD University Hospital, West Java, Indonesia.
Abstract
UC-MSCs appear to be a viable option for stem cell-based treatment. Cynomolgus monkeys, with their psychological and genetic parallels to humans, are essential models for medical study and development. Hypoxia is a basic characteristic of angiogenesis, glucose metabolism, and cell proliferation and survival in both healthy and pathological individuals. This study aimed to develop and characterize UC-MSCs requirements derived from the cynomolgus monkey. We have successfully developed UC-MSCs derived from Mf under hypoxic conditions. Mf UC-MSCs was cultured from the WJ area. Our Mf UC-MSCs have developed into adipocytes, osteocytes, and chondrocytes. The protein of the biomarker MSC was expressed in our hypoxic precondition Mf UC-MSCs and the mRNA expression levels of ERK1/2, AKT1, and HIF-1 alpha increased in the hypoxic condition compared to the normoxic and control groups. The JNK and NFκB genes were decreased in the hypoxic group. Interestingly, our hypoxic Mf UC-MSCs suppressed IL-6 expression. These findings show that preconditioned Mf UC-MSCs produce anti-inflammatory properties, making them potential candidates for regenerative medicine. Their response to hypoxia is crucial for developing targeted therapies to enhance regenerative capabilities in conditions such as ischemic injury and neurodegeneration.
MeSH
Cells, Genes, Microscopy, Electron, Scanning Transmission, Stem Cells, Mesenchymal Stem Cells, Hypoxia, Research, Therapeutics, Umbilical Cord