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Publications

Journal Article Epublish

PubMed: 42079714 DOI: 10.7717/peerj.21128

The oral maternal microbiome plays a role in the development of cleft lip and palate condition in children.

PeerJ 14:e21128

Authors: Suzana Eiko Sato Guima, Bárbara Bischain, Lívia C Morais Gama, Agatha Cristhina Faria, Talita Lourenço, Daniela Franco Bueno, Debora Heller, Maria Rita Passos-Bueno, João C Setubal

Affiliations

  • Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
  • Centro de Estudos do Genoma Humano, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.
  • Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
  • Albert Einstein Israeli College of Health Sciences, Albert Einstein Hospital, São Paulo, Brazil.
  • UT Health San Antonio, University of Texas, San Antonio, TX, United States of America.
  • Universidade Cruzeiro do Sul, São Paulo, Brazil.

Abstract

Non-syndromic cleft lip or palate (NS-CL/P) is an oral birth defect with complex aetiology. We compared the microbial diversity and composition of the oral microbiome of mothers of babies with NS-CL/P (CLP group) and mothers of babies without NS-CL/P (control group). Oral microbiome composition was determined by sequencing the V3-V4 regions of the 16S rRNA gene. CLP and control groups had overall similar microbial compositions, but significant differences were observed. The most significant microbial genus related to these differences was Cutibacterium, which was more abundant in the CLP group. Based on the literature, we hypothesize that a member of the Cutibacterium genus present in the oral microbiota may have a role in inflammation processes that could be related to NS-CL/P development. We found additional differences in terms of differential abundance when subsetting the dataset for mothers with a male child; in this case, depletion of Limosilactobacillus and an unknown taxon, in the CLP group, was a significant result. We conclude that the maternal oral microbiome likely plays a role in the development of the NS-CL/P condition.

MeSH

Genes, Rrna, Mothers, Cleft Lip, Palate, Child, Lip, Oral Microbiome