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Publications

Journal Article Epublish

PubMed: 42088272 DOI: 10.3389/fmicb.2026.1793713

Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments.

Front Microbiol 17:1793713

Authors: Inmaculada Tocino-Márquez, Martin Zehl, Jovana Batajic, Joana Séneca, Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N Sekurova, Sergey B Zotchev

Affiliations

  • Division of Pharmacognosy, Department of Pharmaceutical Sciences, University of Vienna, Vienna, Austria. Doctoral School in Microbiology and Environmental Science, University of Vienna, Vienna, Austria.
  • Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.
  • Institute of Science and Technology Austria, Klosterneuburg, Austria.
  • Division of Pharmacognosy, Department of Pharmaceutical Sciences, University of Vienna, Vienna, Austria.
  • Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria.
  • Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Vienna, Austria.
  • Fundación MEDINA, Armilla, Granada, Spain.

Abstract

Several Streptomyces strains were isolated from freshwater sediments collected in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses revealed biosynthetic gene clusters (BGCs) that may specify production of chemically diverse secondary metabolites. Various culture conditions were employed to induce metabolite production, and subsequent LC-MS analyses facilitated the identification of the produced compounds and their correlation with the corresponding BGCs. These analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic potential, revealing a diverse range of bioactive secondary metabolites, including siderophores, antibiotics, and other compounds with potential therapeutic applications. Genomes of two Streptomyces isolates, one of them representing a potentially new species, harbored several uncharacterized BGCs that may specify biosynthesis of novel secondary metabolites. Although targeted overexpression of pathway-specific regulators from these BGCs did not yield additional metabolites, whereas knockout experiments led to metabolic changes, presumably reflecting regulatory or compensatory interactions between multiple biosynthetic pathways. Continued exploration of these strains and their BGCs may lead to the discovery of new bioactive molecules with pharmaceutical and biotechnological applications.

MeSH

Genome, Fresh Water, Streptomyces