Journal Article Ppublish
PubMed: 42096155 DOI: 10.1007/978-1-0716-5328-9_11
In Vitro Measurement of Clostridioides difficile Biofilm Formation Induced by Gut and Microbiota-Derived Signals.
Methods Mol Biol 3046:147-157
Authors: Carolina Altaf Figueiredo, Bruno Dupuy
Affiliations
- Institut Pasteur, Université Paris-Cité, UMR-CNRS 6047, Laboratoire Pathogenèse des Bactéries Anaérobies, Paris, France.
- Institut Pasteur, Université Paris-Cité, UMR-CNRS 6047, Laboratoire Pathogenèse des Bactéries Anaérobies, Paris, France.
Abstract
Studying biofilm formation in liquid cultures is a rapid and straightforward method for identifying relevant experimental and bacterial factors before conducting tests in more complex gut models. Recent studies showed that several recurrent pathogenic bacteria form biofilms in response to host and microbiota-derived signals. Clostridioides difficile biofilms present as heterogeneous structures are highly dependent on strain, environmental signals, and surrounding microbes. Here, we describe protocols for studying induced C. difficile biofilm formation in the function of gut-derived factors.
MeSH
Research Design, Clostridioides, Biofilms, Clostridioides Difficile, Bacteria, Microbiota