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Publications

Journal Article Ppublish

PubMed: 42160953 DOI: 10.1016/j.biomaterials.2026.124309

One-step prepared janus cryogel enables the separation of blood cells and plasma for whole-process hemostasis and accelerated infected wound healing.

Biomaterials 334:124309

Authors: Kexin Zhang, Haoyan Yang, Tian Sun, Tianbao Li, Xiaohua He, Huixin Yu, Jinlian Hua, Mao-Sen Yuan, Jinyi Wang, Qin Tu

Affiliations

  • College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China; Shanxi Province Key Lab of Plant Extraction and Health of Lujiu, Fenyang, Shanxi, 032205, China.
  • College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Abstract

Uncontrolled hemorrhage continues to be a primary cause of clinical mortality. This study presents the synthesis of a Janus Cryogel (SMCP@MS), which integrates rapid hemostatic properties and promotes the healing of infected wounds, targeting three critical stages of hemostasis. The synthesis employs a settling method, whereby silk fibroin (SF) undergoes conformational transformation and separation in a single step, resulting in a bilayer structure. MXene and hydrophobically modified SF spontaneously settle to form a hydrophobic layer. Upon application to bleeding sites, the hydrophobic layer effectively intercepts blood cells, while plasma moves unidirectionally into the material, leading to the separation of plasma from blood cells, which facilitates the second stage of hemostasis by promoting platelet adhesion and thrombus formation. The cross-linked hydrophilic SF forms a macroporous structure with continuous fluid absorption capacity, enabling sustained enrichment of blood components and facilitating the formation of stable fibrin networks, corresponding to the third stage of hemostasis. Furthermore, carbonized typhae pollen (CP) enhances the first stage of hemostasis by expediting vasoconstriction. In an infected wound model, SMCP@MS demonstrated a significantly greater efficacy in wound healing compared to commercial dressings, underscoring its high potential as a dressing, particularly for the treatment of non-compressible hemorrhage and traumatic infections.

MeSH

Cells, Hemorrhage, Blood, Fibroins, Biocompatible Materials, Blood Cells, Cryogels, Hemostasis, Plasma