Journal Article Epublish
PubMed: 42187659 DOI: 10.3390/dj14050281
The Effects of Immediate Dentin Sealing on the Enzymatic Activity and Bond Strength of Hybrid Ceramic CAD/CAM Onlays: A Comparative Study of Two Universal Adhesives.
Dent J (Basel) 14(5)
Authors: Uros Josic, Tatjana Maravic, Carlo D'Alessandro, Annamaria Forte, Diego D'Urso, Sofia Avnet, Edoardo Mancuso, Annalisa Mazzoni, Claudia Mazzitelli, Lorenzo Breschi
Affiliations
- Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, Italy.
Abstract
Background/Objectives: To elucidate the effects of immediate dentin sealing (IDS) with two universal adhesive systems on the microtensile bond strength (µTBS) and enzymatic activity (MMPs) of CAD/CAM hybrid ceramic onlays. Methods: Twenty-four human molars were assigned to one of the following groups (n = 8) according to IDS and the adhesives used: (1) Clearfil Universal Bond Quick (IDS QB; Kuraray); (2) Scotchbond Universal Plus (IDS SB; 3M); (3) no IDS (CTR). CAD/CAM onlays (Katana Avencia Block, Kuraray) were luted using a silane-containing universal composite cement (Panavia SA Cement Universal, Kuraray). µTBS tests and scanning electron microscope (SEM) analysis were performed after 24 h (T 0) or 10,000 thermocycles (T t). Three additional molars per group were processed to evaluate the effects of IDS on MMPs using in situ zymography at T 0 and T t. Data were statistically analyzed (α = 0.05). Results: At T 0, IDS QB showed a significantly higher µTBS than IDS SB and CTR (p < 0.05). Artificial aging led to significant reductions in µTBS in IDS QB and CTR (p < 0.05), while µTBS remained stable in IDS SB. Both IDS groups demonstrated higher µTBS compared with CTR after aging (p < 0.05). At T 0, the application of a universal adhesive system significantly increased the levels of MMPs (IDS QB > IDS SB > CTR; p < 0.05). At T t, IDS QB exhibited higher MMP activity compared with CTR (p < 0.05). Conclusions: IDS had a positive effect on immediate and aged µTBS, while the level of MMP activity was found to be material-dependent.
MeSH
Molar, Inlays, Aging, Ceramics, Dentin, Adhesives