Journal Article Ahead of print
PubMed: 42223859 DOI: 10.1186/s41181-026-00452-0
Development of an automated radiosynthesis for [ 68Ga]Ga-NODAGA[40Lys]-Exendin-4 in sodium acetate buffer.
EJNMMI Radiopharm Chem
Authors: Maïlys Ragot, Pauline Reynaud-Orhon, Alban Revy, Marie Batisse-Lignier, Valentin Brusseau, Antony Kelly, Hosameldin Otman, Florent Cachin, Marion Chanchou, Sophie Levesque, Marion Tempier
Affiliations
- Radiopharmacy, Jean Perrin Center, 58, Rue Montalembert, 63005, Clermont, Ferrand, France.
- Nuclear Medicine, Jean Perrin Center, 58, Rue Montalembert, 63005, Clermont, Ferrand, France.
- Molecular Imaging and Theranostic Strategies, UMR 1240, Clermont Auvergne University (UCA), INSERM, 58, Rue Montalembert, 63005, Clermont, Ferrand, France.
- Radiopharmacy, Jean Perrin Center, 58, Rue Montalembert, 63005, Clermont, Ferrand, France.
- Molecular Imaging and Theranostic Strategies, UMR 1240, Clermont Auvergne University (UCA), INSERM, 58, Rue Montalembert, 63005, Clermont, Ferrand, France.
Abstract
BACKGROUND
[ 68Ga]Ga-NODAGA[40Lys]-Exendin-4 ( 68Ga-Ex-4) is a new PET radiopharmaceutical targeting GLP-1-R that showed great specificity and sensitivity in the topographic diagnosis of benign insulinoma. We herein report the development of a new method for 68Ga-Ex-4 radiolabeling in sodium acetate buffer, automated on the miniAllinOne synthesizer.
RESULTS
Key parameters were reaction volume, due to low amount of peptide, and pH. At a reaction volume of 2.65 mL and pH 2.9, the reaction yielded 80% of 68Ga-Ex-4. The use of polysorbate and cooling of the solution allows to decrease the adsorption on the reaction vial (from 29 to 2% of total activity remaining on the vial). 68Ga-Ex-4 is purified on a HLB cartridge. Final product was obtained within 40 min, with a mean radiochemical purity of 97% and a radiochemical yield of 67%.
CONCLUSIONS
We have successfully developed a new radiolabeling method for [ 68Ga]Ga-NODAGA-[40Lys]Exendin-4, automated on a miniAllinOne synthesizer and using sodium acetate buffer, suitable for clinical application.
MeSH
Sodium Acetate, Buffers, Acetates