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Publications

Journal Article Epublish 1 citation

PubMed: 42347266 DOI: 10.3390/pathogens15060654

Molecular Characterization of Hemotropic Mycoplasma spp. From Bats (Chiroptera) in the Northern Pantanal, Brazil.

Pathogens 15(6)

Authors: Nathalia de Assis Pereira, Juliane Saldanha, Thiago Borges Fernandes Semedo, Guilherme Siniciato Terra Garbino, Rogério Vieira Rossi, Sofia de Souza Pereira Gomes, Sayanne Luns Hatum, Thállitha Samih Wischral Jayme Vieira, Rafael Felipe da Costa Vieira, Jansen de Araújo, Edison Luiz Durigon, Daniel Moura de Aguiar

Affiliations

  • Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiabá 78060-900, Brazil.
  • CIBIO, Centro de Investigacão em Biodiversidade e Recursos Genéticos, Universidade do Porto, 4099-002 Porto, Portugal.
  • Departamento de Biologia, Universidade do Porto, 4099-002 Porto, Portugal.
  • BIOPOLIS Program in Genomics, Biodiversity and Land Planning, 4099-002 Porto, Portugal.
  • Museu de Zoologia João Moojen, Departamento de Biologia Animal, Universidade Federal de Vicosa, Viçosa 36570-900, Brazil.
  • Laboratory of Mastozoology, BioScience Institute, Federal University of Mato Grosso State, Cuiabá 78060-900, Brazil.
  • Department of Chemistry, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
  • Department of Epidemiology and Community Health, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
  • Center for Computational Intelligence to Predict Environment and Health Risks, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
  • Emerging Viruses Research and Clinical and Molecular Virology Laboratories, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Institut Pasteur of São Paulo, São Paulo 05508-020, Brazil.

Abstract

In this study, we conducted a molecular investigation of hemotropic Mycoplasma spp. in bat species captured in the northern region of the Pantanal biome, Mato Grosso State, Brazil. Tissue samples were screened by qPCR targeting the 16S rRNA gene. Positive samples were subsequently subjected to conventional PCR assays targeting partial fragments of the 16S rRNA (~900 bp) and 23S rRNA (~800 bp) genes. Hemoplasma DNA was detected in four bat species: Glossophaga soricina, Molossops temminckii, Molossus rufus, and Desmodus rotundus. Phylogenetic analyses based on partial 16S and 23S rRNA gene sequences demonstrated that the detected hemoplasmas clustered predominantly with previously described bat-associated hemoplasmas from Brazil and other countries in the Americas. Notably, the detection in M. temminckii represents, to our knowledge, the first molecular evidence of hemotropic Mycoplasma infection in this bat species. These findings expand current knowledge regarding the occurrence, host range, and genetic diversity of hemotropic Mycoplasma spp. in bats from the Pantanal biome and contribute to wildlife surveillance efforts in this ecologically important region.

MeSH

Genes, Genes, Rrna, Mycoplasma