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Publications

Journal Article Ahead of print

PubMed: 42409147 DOI: 10.1016/j.ijbiomac.2026.153322

Proteomic characterization of cellular responses to the fish allergen β-parvalbumin using ZenoSWATH-MS.

Int J Biol Macromol

Authors: Robert Stryiński, Jesús Mateos, Javier Freire González, África González-Fernández, Ignacio Ortea, Manuel Pazos, Susana Magadán, Mónica Carrera

Affiliations

  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego Str. 2, 10-719, Olsztyn, Poland; Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo, Lagoas Marcosende, 36-310, Vigo, Pontevedra, Spain.
  • Clinical Research Unit/GIOT-HULA, Department of Medical Oncology, Lucus Augusti University Hospital, Lugo, 27-003, Spain.
  • Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo, Lagoas Marcosende, 36-310, Vigo, Pontevedra, Spain.
  • Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo, Lagoas Marcosende, 36-310, Vigo, Pontevedra, Spain; Immunology Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, 36-312, Vigo, Pontevedra, Spain.
  • Proteomics Unit, Nanomaterials and Nanotechnology Research Center (CINN), Spanish National Research Council (CSIC), and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Avda. del Hospital Universitario, 33-011, Oviedo, Spain.
  • Department of Food Technology, Institute of Marine Research (IIM-CSIC), Spanish National Research Council (CSIC), Eduardo Cabello 6, 36-208, Vigo, Pontevedra, Spain.
  • Immunology Group, Center for Research in Nanomaterials and Biomedicine (CINBIO), University of Vigo, Lagoas Marcosende, 36-310, Vigo, Pontevedra, Spain; Immunology Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, 36-312, Vigo, Pontevedra, Spain.
  • Department of Food Technology, Institute of Marine Research (IIM-CSIC), Spanish National Research Council (CSIC), Eduardo Cabello 6, 36-208, Vigo, Pontevedra, Spain.

Abstract

β-Parvalbumin (β-PRVB) is a calcium-binding protein and the major allergenic macromolecule responsible for most IgE-mediated fish allergy reactions. Despite its clinical relevance, the molecular mechanisms underlying interactions between β-PRVB and host cellular systems remain incompletely understood. In this study, high-resolution quantitative proteomics using data-independent acquisition ZenoSWATH-MS was applied to investigate cellular responses associated with exposure to β-PRVB in a controlled experimental model. Proteomic profiling of sensitized splenocytes following ex vivo stimulation with β-PRVB quantified 4784 proteins and identified 324 differentially regulated proteins. Functional enrichment analysis linked these changes to antigen processing and immune signaling pathways. Notably, β-PRVB exposure was associated with coordinated upregulation of immunoproteasome subunits together with components of the JAK/STAT and Notch signaling pathways. These results indicate that β-PRVB modulates intracellular protein networks involved in antigen processing and immune activation. This proteomic characterization provides molecular insight into the biological activity of the fish allergen β-parvalbumin and highlights cellular pathways associated with its allergenic potential.

MeSH

Parvalbumins, Allergens