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Publications

Journal Article Ahead of print 1 citation

PubMed: 42439832 DOI: 10.1097/SCS.0000000000013131

Aloin as a Preclinical Candidate for Wound Healing: Evidence From Fibroblast Cell Culture.

J Craniofac Surg

Authors: Nurcan Yoruk, Nuray Bayar Muluk, Şeyma Nur Dere, Talat Yasin Acar, Suat Utku Keskin, Cemal Cingi

Affiliations

  • Department of Obstetrics and Gynecology, University of Health Sciences, Erzurum City Hospital, Erzurum.
  • Department of Otorhinolaryngology, Faculty of Medicine, Kırıkkale University, Kırıkkale.
  • SABITA Research Institute for Health Sciences and Technologies, Istanbul Medipol University.
  • Department of Histology and Embryology, School of Medicine, Istanbul Medipol University.
  • School of Medicine, Koc University, Istanbul.
  • Department of Otorhinolaryngology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir.
  • Department of Otorhinolaryngology, Faculty of Medicine, Biruni University, Istanbul, Turkey.

Abstract

OBJECTIVES

The antioxidant, anti-inflammatory, and skin-protective characteristics of aloin, a bioactive anthraquinone glycoside extracted from Aloe vera, have garnered significant interest. Its compatibility with dermal fibroblasts is an important prerequisite for its use in wound-healing formulations. To establish a safe in vitro concentration range for future wound-healing research, this work assessed the cytotoxic effects of aloin on primary human dermal fibroblast cells, which vary with concentration.

METHODS

We cultured primary human adult dermal fibroblasts (HDFa) according to standard protocols after obtaining them from ATCC. Various concentrations of aloin (10, 25, 50, 100, 150, and 200 µM) were applied to the cells. The MTT colorimetric test was used to determine the cell viability. A positive cytotoxic control was used with Triton X-100, whereas untreated cells grown in DMEM served as the negative control. The statistical analyses were carried out using GraphPad Prism 10, namely one-way ANOVA and nonlinear regression analysis.

RESULTS

No concentration of aloin treatment significantly decreased fibroblast vitality compared with the control group (P>0.05). The lack of a concentration-dependent cytotoxic response was demonstrated by nonlinear regression analysis up to 200 µM. Triton X-100, in contrast, significantly reduced cell viability, thereby demonstrating the assay's experimental validity and sensitivity.

CONCLUSION

In vitro biocompatibility studies with primary human dermal fibroblasts showed that aloin was very safe and did not cause cytotoxicity at the concentrations considered. Based on these results, aloin appears to be a good choice for future preclinical research on skin regeneration and wound healing.

MeSH

Regression Analysis, Skin, Cells, Cell Survival, Research, Fibroblasts, Cell Culture Techniques