Journal Article Evaluation Study Epublish
PubMed: 42585413 DOI: 10.1590/1807-3107bor-2026.vol40.053
In vitro and in vivo biocompatibility and bioactivity of bioceramic repair materials.
Braz Oral Res 40:e053
Authors: Lara Cancella de Arantes, Alexandre Henrique Dos Reis-Prado, Karina Sampaio Caiaffa, Pedro Augusto Monteiro Delgado, Sabrina de Castro Oliveira, Francielen Oliveira Fonseca, Warley Luciano Fonseca Tavares, Cristiane Duque, Luciano Tavares Angelo Cintra, Carlos Roberto Emerenciano Bueno, Ricardo Alves de Mesquita, Gustavo Batista de Menezes, Antônio Paulino Ribeiro-Sobrinho, Francine Benetti
Affiliations
- Universidade Federal de Minas Gerais - UFMG, School of Dentistry, Department of Restorative Dentistry, Belo Horizonte, MG, Brazil.
- Universidade Estadual Paulista - Unesp, School of Dentistry, Department of Pediatric Dentistry and Public Health, Araçatuba, SP, Brazil.
- Universidade Católica Portuguesa - UCP, Faculty of Dental Medicine, Centre for Interdisciplinary Research in Health, Viseu, Portugal.
- Universidade Estadual Paulista - Unesp, School of Dentistry, Department of Restorative Dentistry, Araçatuba, SP, Brazil.
- Universidade Federal de Minas Gerais - UFMG, School of Dentistry, Department of Dental Clinic, Pathology and Surgery, Belo Horizonte, MG, Brazil.
- Universidade Federal de Minas Gerais - UFMG, Institute of Biological Sciences Department of Morphology, Belo Horizonte, MG, Brazil.
Abstract
This study aimed to evaluate the in vitro cytotoxicity and bioactivity, and the in vivo biocompatibility, collagen maturation, and bioactivity, of PBS Cimmo HP and Bio-C Repair Ion+ in comparison with white MTA-Angelus. Osteoblast-like cell viability was assessed using the Alamar Blue assay, while bioactivity was assessed by alkaline phosphatase (ALP) activity and Alizarin Red staining. For the in vivo analysis, polyethylene tubes containing the materials or empty tubes (control) were implanted in the dorsum of mice. After 7 and 30 days (n = 8), the animals were euthanised and the specimens were processed for haematoxylin-eosin, Picrosirius Red, von Kossa (VK), and polarised light (PL) analyses. Data were statistically analysed (p < 0.05). Most materials extracts were cytocompatible (P > 0.05), except for the PBS Cimmo HP 1:4 extract at 48-h (p < 0.05). All materials showed increased ALP activity (p < 0.05). Mineralized nodules were significantly present in most material extracts (p < 0.05), except in the undiluted PBS Cimmo HP extract and in the white MTA-Angelus 1:4 extract. At 7 days, PBS Cimmo HP induced moderate-to-severe inflammation, whereas the other groups induced moderate inflammation (p > 0.05). At 30 days, all groups showed reduced inflammation (p > 0.05). Overall, the fibrous capsule was thick at 7 days and thin at 30 days. At 7 days, the control and PBS Cimmo HP groups showed a greater presence of immature collagen (p < 0.05); at 30 days, more mature collagen was observed in all groups (p > 0.05). The materials exhibited positivity for VK or PL in both periods. In conclusion, PBS Cimmo HP and Bio-C Repair Ion+ were cytocompatible and biocompatible, and demonstrated bioactivity and collagen maturation similar to those of white MTA-Angelus.
MeSH
Dentistry, Collagen