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Publications

Journal Article Epublish

PubMed: 42614915 DOI: 10.1002/rmb2.70082

The Vitamin D Analogs Doxercalciferol Induces Weight Loss and Improves Fertility in a Mouse Model of Polycystic Ovary Syndrome.

Reprod Med Biol 25(1):e70082

Authors: Hang-Soo Park, Ana Corachan, Jin Seok, Farzana Liakath Ali, Mohammad Mousaei Ghasroldasht, Safia Akhtar, Hanaa Mohammed, Charlotte Manier, Abdullah Awartani, Nahed Ismail, Hyunho Kim, Ayman Al-Hendy

Affiliations

  • Department of Biomedical Science Sunchon National University Suncheon Republic of Korea. Research Institute of Basic Sciences Sunchon National University Suncheon Republic of Korea. Department of Obstetrics and Gynecology University of Chicago Chicago Illinois USA.
  • Department of Obstetrics and Gynecology University of Chicago Chicago Illinois USA.
  • IVI Foundation, Instituto de Investigación Sanitaria La Fe Valencia Valencia Spain.
  • Human Anatomy and Embryology Department, Faculty of Medicine Sohag University Sohag Egypt.
  • Department of Basic Medical Sciences Al Rayan National College of Medicine Madina Saudi Arabia.
  • Department of Obstetrics and Gynecology University of Chicago Chicago Illinois USA. Biological Science Division University of Chicago Chicago Illinois USA.
  • Department of Obstetrics and Gynecology University of Chicago Chicago Illinois USA. College of Medicine King Saud University Riyadh Saudi Arabia.
  • Department of Pathology, College of Medicine University of Illinois at Chicago Chicago Illinois USA.
  • Dept of Medical Sciences Khalifa University Abu Dhabi UAE.

Abstract

PURPOSE

Polycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder in reproductive-age women. Several published studies demonstrated the therapeutic potential of vitamin D on PCOS condition, but vitamin D supplementation leads to only modest improvements. Doxercalciferol (Dox) is a highly potent vitamin D receptor (VDR) agonist that binds to VDR with at least 150 times greater affinity than other vitamin D compounds. In this study, we hypothesized that the therapeutic potential of Dox may reverse PCOS-related phenotypes more efficiently.

METHOD

We first analyzed the regulatory potential of Dox in fat metabolism by comparing adipocyte proliferation and differentiation in our in vitro model. We also injected Dox into our mouse model of PCOS to analyze the therapeutic effect of Dox treatment on various PCOS-related parameters and fertility.

RESULT

Dox treatment inhibited the proliferation of both adipose lineage cells and androgen-producing H295R cells in a dose-dependent manner. PCOS-related outcomes such as body weight and fertility rate were significantly reversed in Dox-treated PCOS mice model.

CONCLUSION

Our study revealed that vitamin D analogs are a promising family of compounds for reducing body weight and reversing infertility in an animal model of PCOS.

MeSH

Vitamin D, Cells, Fertility, Vitamins, Ovary, Body Weight, Obesity, Polycystic Ovary Syndrome, Weight Loss, Therapeutics