Journal Article Epublish
PubMed: 42624939 DOI: 10.1007/s00299-026-03913-7
The Arabidopsis tex1-4 mutant exhibits altered mRNA export and hormonal crosstalk in plant immunity against Pseudomonas syringae.
Plant Cell Rep 45(9)
Authors: Shahab Uddin, Kris John Silvano, Rupak Chakraborty, Donah Mary Macoy, Gyeong Ryul Ryu, Rakhimov Abdurakhim Ramazon Ugli, Gyeongik Ahn, Hamad Khan, Joon-Yung Cha, Woe-Yeon Kim, Min Gab Kim
Affiliations
- College of Pharmacy and Research Institute of Pharmaceutical Science, PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
- Division of Applied Life Science (BK21 Four), PMBBRC, RILS & IALS, Gyeongsang National University, Jinju, 52828, Republic of Korea.
- Plant Biological Rhythm Research Center, Graduate School of Gyeongsang National University, Jinju, 52828, Republic of Korea.
- Department of Biological Science, University of Arkansas, Fayetteville, AR, 72703, USA.
- Division of Applied Life Science (BK21 Four), PMBBRC, RILS & IALS, Gyeongsang National University, Jinju, 52828, Republic of Korea. Plant Biological Rhythm Research Center, Graduate School of Gyeongsang National University, Jinju, 52828, Republic of Korea.
- Division of Applied Life Science (BK21 Four), PMBBRC, RILS & IALS, Gyeongsang National University, Jinju, 52828, Republic of Korea. Plant Biological Rhythm Research Center, Graduate School of Gyeongsang National University, Jinju, 52828, Republic of Korea.
- College of Pharmacy and Research Institute of Pharmaceutical Science, PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea. Kims BioBiotech Inc. Jinju, 52828, Republic of Korea.
Abstract
TEX1 contributes to mRNA export and SA-mediated defense while modulating ABA-driven susceptibility, linking RNA processing to immune regulation in Arabidopsis TEX1, a subunit of the multi-protein THO/TREX complex conserved in eukaryotes, facilitates the synthesis, splicing, and nuclear-cytoplasmic export of mRNAs. However, its mechanism in plants remains to be thoroughly established, particularly its contribution to plant immunity and the mRNA export mechanism in response to pathogen challenges. Here, we showed that TEX1 contributes to mRNA export and hormonal signaling during pathogen infection. The TEX1 mutant was impaired in plant resistance to the Pseudomonas syringae pv. tomato DC3000 and exhibited a compromised salicylic acid (SA) accumulation and reduced expression of SA-regulated genes. Moreover, we show that the pattern-triggered immunity (PTI) response was compromised in the tex1-4 mutant, as it contributes to a reduced defense phenotype upon flg22, leading to reduced flg22-induced defense responses. TEX1 also positively regulates effector-triggered immunity (ETI) against Pto carrying AvrRpm1 and AvrRpt2. Epistasis analysis revealed that TEX1-mediated resistance is SA-dependent. The tex1-4 mutant accumulated elevated abscisic acid (ABA) levels and increased ABA-mediated susceptibility, accompanied by enhanced ABA biosynthesis and reduced expression of catabolic genes. Analysis with sid2-2 further indicated that ABA-induced susceptibility depends on SA signaling, supporting SA-ABA antagonism. Furthermore, the tex1-4 mutation exhibited defective bulk nuclear mRNA export to the cytoplasm upon pathogen infection, as observed by in situ hybridization using 48-mer fluorescein-labeled oligo(dT) probe. Thus, our findings suggest that mRNA export is a crucial component of plant immune activation, with TEX1 serving as a contributing factor that mechanistically links mRNA export with hormone-regulated immune signaling to promote effective plant defense.
MeSH
Genes, Pseudomonas Syringae, Pseudomonas, Rna, Messenger, Plant Immunity, Cytoplasm, Immunity, Infections