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Publications

Journal Article Ppublish 1 citation

PubMed: 42664039 DOI: 10.1073/pnas.2525536123

PLASTID ENVELOPE ION CHANNELS (PEC1/2) link Ca 2+ and jasmonic acid signaling in plant cells.

Proc Natl Acad Sci U S A 123(35):e2525536123

Authors: Susanne Mühlbauer, Dawid Jaślan, Inês F Duarte Nunes, Benjamin Brandt, Lena Wutz, Khansa Mekkaoui, Sanja Zenker, Jakob Rehberger, Marlena Rädler, Lorenz Holzner, Constance Tisserant, Carsten Völkner, Andrea Bräutigam, Silke Robatzek, Bettina Hause, Christian Grimm, Hans-Henning Kunz

Affiliations

  • Plant Biochemistry and Physiology, Faculty of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried 82152, Germany.
  • Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, Ludwig-Maximilians-University Munich, Munich 80336, Germany.
  • Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle/Saale 06120, Germany.
  • Computational Biology, Faculty of Biology, Bielefeld University, Bielefeld 33615, Germany.
  • Department of Computational Biology, Center of Biotechnology, Bielefeld University, Bielefeld 33615, Germany.
  • Genetics, Faculty of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried 82152, Germany.
  • Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, Ludwig-Maximilians-University Munich, Munich 80336, Germany. Department of Pharmacology, Faculty of Medicine, University of Oxford, Oxford OX1 3QT, United Kingdom. Immunology, Infection and Pandemic Research, Fraunhofer Institute for Translational Medicine and Pharmacology, Munich/Frankfurt 80799, Germany.

Abstract

Plants constantly encounter adverse environmental interactions. One organelle is particularly specialized in stress signaling and phytohormone synthesis: the plastid. Calcium (Ca 2+), a key second messenger, is known to intersect with cellular phytohormone signaling networks. While cytosolic Ca 2+ dynamics have been studied extensively, the physiological relevance of stromal Ca 2+ transients and the identity of channels mediating rapid Ca 2+ flux into plastids remain largely unexplored. In this study, we provide evidence for PLASTID ENVELOPE ION CHANNELS (PECs) as long-sought mediators of fast-activating cation channel-like currents. We show that PEC expression is jasmonic acid (JA)-induced to augment stromal Ca 2+ transients under stress. Loss of PECs results in decreased JA priming and failure to elicit full defense responses after wounding. In turn, PEC1 overexpression improves Botrytis cinerea tolerance. Our findings link stromal Ca 2+ signaling with JA-dependent stress responses and position PECs as modulators of plant defense.

MeSH

Cells, Stress, Physiological, Chloroplasts, Ion Channels, Plant Growth Regulators, Plastids, Plant Cells