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Publications

Journal Article Ppublish

PubMed: 42685185 DOI: 10.1126/sciadv.aeh7630

AKT5 is a bona fide potassium channel and controls petiole growth in Arabidopsis.

Sci Adv 12(36):eaeh7630

Authors: Yuki Muraoka, Shunsuke Kobayashi, Lisa Oskam, Michihiro Tateyama, Ayumu Masago, Yoshikazu Tanaka, Takeshi Yokoyama, Tadaomi Furuta, Hinano Takase, Ellen Tanudjaja, Shota Terashima, Haruto Shimizukawa, Taro Yamanashi, Shunya Saito, Hiroki Ijima, Miyuki Tanaka, Atsushi Miyamoto, Megumi Kato, Kanane Sato, Masaru Tsujii, Taishi Umezawa, Francisco J Quintero, Francisco Rubio, Jörg Kudla, Masaaki Ito, Yoshihiro Kubo, Yasuhiro Ishimaru, Ronald Pierik, Nobuyuki Uozumi

Affiliations

  • Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University; Sendai, 980-8579, Japan.
  • Laboratory of Molecular Biology, Wageningen University and Research; Wageningen, 6708 TB, The Netherlands.
  • Division of Biophysics and Neurobiology, Department of Molecular and Cellular Physiology, National Institute for Physiological Sciences; Okazaki, 444-8585, Japan.
  • Department of Molecular and Chemical Life Sciences, Graduate School of Life Sciences, Tohoku University; Sendai, 980-8577, Japan.
  • School of Life Science and Technology, Institute of Science Tokyo; Yokohama, 226-8501, Japan.
  • Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology; Koganei, 184-8588, Japan.
  • Institute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas and University of Seville; Seville, 41092, Spain.
  • Departamento de Nutrición Vegetal, CEBAS-CSIC, Campus de Espinardo; Murcia, 30100, Spain.
  • Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster; Münster, 48149, Germany.
  • Department of Bioresources Engineering, National Institute of Technology, Okinawa College; Nago, 905-2192, Japan.

Abstract

Potassium ion (K +) is essential for plant growth and development. Despite decades of study, the Shaker-type K + channel AKT5 has remained functionally unassigned. Here, we report that AKT5 functions as a bona fide voltage-dependent K + channel and serves as an essential regulator of petiole elongation. Structural analyses of AKT5 in a closed state and the AKT5-D403 variant in a pre-open state provide direct structural evidence for functional K + channel activity. AKT5 assembled into a canonical tetramer with two-fold symmetry and, upon phosphorylation, operated as an inward-rectifying K + channel activated at strongly hyperpolarized membrane potentials. AKT5 was predominantly expressed in young petioles, and loss of AKT5 function largely suppressed petiole elongation throughout the diurnal cycle, resulting in compact rosettes and reduced biomass under crowded growth conditions. These findings show that AKT5 is involved in a regulator of plant competitive growth and high-density performance.

MeSH

Growth, Biological Science Disciplines, Potassium Channels