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Publications

Journal Article Epublish

PubMed: 42699323 DOI: 10.1016/j.fochms.2026.100448

Tissue-specific transcriptomic responses and skeletal-muscle ultrastructural observations in Semah mahseer (Tor douronensis) under acute thermal and hypoxic conditions.

Food Chem (Oxf) 13:100448

Authors: Anurak Uchuwittayakul, Witchukorn Phuthong, Chamroeun Kong, Chonlatat Rajitdumrong, Yosapon Adisornprasert, Benchawan Kumwan, Pakapon Meachasompop, Salsa Safitri Putri Ely, Kanithaporn Vangnai, Kornsorn Srikulnath, Prapansak Srisapoome

Affiliations

  • Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Special Research Incubator Unit for Development and Application of Vaccine Delivery Systems for Aquatic Animals, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Department of Physics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
  • Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand. Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand. Special Research Incubator Unit for Development and Application of Vaccine Delivery Systems for Aquatic Animals, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Master Program in Science for Industry, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
  • Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
  • Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.

Abstract

Semah mahseer (Tor douronensis) is a high-value freshwater cyprinid native to Southeast Asia. This study characterized acute transcriptomic responses in liver and white skeletal muscle following 6 h exposure to hyperthermia (35 °C), hypoxia (dissolved oxygen 2 mg/L), or their combination. Thirty-two RNA-seq libraries yielded approximately 48,504 annotated unigenes. Relative to the control, hyperthermia produced 7776 differentially expressed genes (DEGs) in liver and 1121 in muscle, hypoxia produced 2451 and 318 DEGs, and combined exposure produced 4952 and 717 DEGs, respectively. The response involved heat-shock proteins, endoplasmic-reticulum protein processing, glycolytic and oxygen-sensing pathways, mitochondrial and contractile programs, and apoptosis-associated signaling. Expression directions for 20 selected transcripts were compared by qRT-PCR; the reported cross-platform correlation was r = 0.91, although interpretation remains conditional on validation of reference-gene stability. Qualitative scanning electron microscopy showed region-dependent disruption of skeletal-muscle organization under the stress treatments. Because the study used one acute endpoint, two replicate tanks per condition, a de novo transcriptome, and no direct postmortem flesh-quality measurements, the findings should be interpreted as mechanistic hypotheses requiring independent validation.

MeSH

Genes, Muscles, Hyperthermia, Liver, Hypoxia, Muscle, Skeletal, Tissues