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OpenAlex: W4387532522 DOI: 10.3892/wasj.2023.206
Integrated molecular docking, dynamic simulations and <i>in vivo</i> analysis of ethanol extract <i>Citrus sinensis</i> peel as an antioxidant and neurotrophic agent for ameliorating motor and cognitive functions in traumatic brain injury
World Academy of Sciences Journal 5(6)
Authors: Widodo Santoso, Gumilar Putra, Mokhamad Syaban, Rossa Vadhana, Jaya Khamid, Iqbal Maududi, Hidayat Sujuti
Affiliations
- Department of Neurology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia
- Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia
- Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia
- Department of Ophthalmology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia
Abstract
Traumatic brain injury (TBI) is the primary injury to the brain caused by mechanical forces. It is one of the leading causes of mortality in mid‑life. Increased levels of inflammation and oxidation, and decreased levels of neurotrophic factor lead to a worsening of the condition. In previous studies, the authors demonstrated that the ethanol extract of Citrus sinensis peel (CSPE) functioned as an antioxidant using molecular docking and dynamic simulations. In the present study, in order to confirm these findings, a Marmarou weight drop model which included rats with TBI. Molecular docking against c‑Jun N‑terminal kinase 3 (JNK3) was also performed. The rats were divided into the sham‑operated, control and treatment groups. The treatment groups received CSPE at 50, 250 and 500 mg/kg body weight (groups 1, 2 and 3, respectively) orally once a day for 7 days following the induction of TBI. Superoxide dismutase (SOD)1, SOD2, and brain‑derived neurotrophic factor (BDNF) expression levels were analyzed using immunohistochemistry. Surviving neurons were analyzed using hematoxylin and eosin staining. Barnes' maze and wire grip tests were performed on the 1st, 3rd and 7th days following TBI. The results revealed a significant increase in the numbers of surviving neurons and in BDNF expression in groups 2 and 3, a higher expression of SOD1 in group 3, and a higher expression of SOD2 in all the treatment groups. Wire grip duration was significantly longer on the 7th day following TBI in group 3, and Barnes maze latency was significantly shorter on the 3rd and 7th days following TBI compared to the previous time in all treatment groups. Molecular docking analysis also revealed that CSPE compounds, particularly chanoclavine and nootkatone, interacted with JNK3 in the same binding pocket. On the whole, the present study demonstrates that CSPE helps protect the brain from damage by functioning as an antioxidant, increasing neurotrophic activities and inhibiting apoptosis. This reduces the overall neurological issues that are associated with TBI. However, further studies on the efficacy and safety of CSPE are required in order to determine its full potential in improving motor and cognitive functions following TBI.
MeSH
Brain-Derived Neurotrophic Factor, Brain Injuries, Brain, Antioxidants, Jnk Mitogen-Activated Protein Kinases, Brain Injuries, Traumatic, Ethanol, Cognition