Brief Summary

This project aims to study the immune responses to peanut allergen in those with a skin barrier defect with and without skin massage, specifically it aims to:

1. Establish if peanut allergen components can pass into human skin through regular massage using the peanut protein-containing extract.
2. Clarify whether this effect is amplified in those with an impaired skin barrier (AD and dry skin vs healthy controls).
3. Assess whether peanut protein components can be detected in interstitial skin fluid (ISF) using a suction device.
4. Test whether peanut protein components present in ISF are able to induce activation of basophils in blood of peanut allergic donors.
5. Assess whether the transcutaneous uptake of peanut protein can be reduced by the prior use of a barrier enhancing cream.

Conditions

• Allergy;Food
• Food Allergy Peanut
• Pathways and Sources of Exposure

Intervention / Treatment

• Other: Application of the barrier enhancing preparation.

Primary Outcome Measures

• Detection of peanut protein components (μg of the proteins per cm² of skin) in retrieved interstitial fluid. (8 weeks)
  Detection of peanut protein components (μg of the proteins per cm² of skin) in retrieved interstitial fluid.
• Activation of blood basophil from peanut allergic donors (measured as %CD63-positive basophils) by peanut proteins present in interstitial fluid. (8 weeks)
  Activation of blood basophil from peanut allergic donors (measured as %CD63-positive basophils) by peanut proteins present in interstitial fluid.

Countries

United Kingdom

Locations and Investigators

• Unit for Population-Based Dermatology Research, London, United Kingdom (Recruiting)
  • Preeti Kaur Khurana, MSc

Study Officials

• Carsten Flohr (Principal Investigator) — King's College London