Jean-Marie Pagès

PAGES Jean-Marie

UMR-MD1, U-1261, Membranes et Cibles Thérapeutiques. Faculté de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille cedex 05, France.

phone secretary office (33) 4 32 45 87; direct (33) 4 91 32 46 97.

jean-marie.pages@univ-amu.fr

Education/Academic degrees and Research Position

1. PhD Microbiology Biochemistry (Immunochemistry of Alkaline Phosphatase), 1975, Université de Provence, Marseille, France
2. Doctorat Es-Sciences (Study of the Biogenesis of E. coli Envelope), 1983, Université de la Méditerranée, Marseille, France
3. 1996-2001, Head of CJF INSERM Bacterial Enveloppe, Permeability and Antibiotics
4. 2004-2012, Head of UMR-MD1 Research Unit "Membrane Transporters, Chemoresistance and Drug design",
5. 2009, Senior Director of Research (DR1) INSERM
6. 2012-2017, Head of UMR-MD1, Unité Mixte de Recherche Ministère de l’Enseignement Supérieur et de la Recherche et Ministère de la Défense) (Joint research Unit)
7. From Sept 2018, Emeritus Director of Research INSERM

Expertises and Scientific Committee

1. Expert for National Science Foundation NSF-USA and NIH-USA; Singapour-National Medical Research Council; USA-Israel Research & Development Fund; Natural and sciences and Engineering Research Council of Canada; Swiss National Science Foundation, Belgium F.R.S-FNRS, 2004-.
2. Expert for 7^th PCRDT EC, H2020, ERC panel.
3. Member of Scientific Committee on Emerging and Newly Identified Health Risks, SCENIHR (EC. DG SANCO) http://ec.europa.eu/health/scientific_committees/emerging/index_en.htm, 2004-2012.(see publications)
4. Member of the Scientific Advisory Committee Synchrotron Soleil, Gif/Yvette, France, 2016-2022.
5. Member of the Scientific Advisory Committee Instituto de Higiene e Medicina Tropical, Lisboa, Portugal, 2015-2022.
6. Feb2020- Feb2024, Scientific Referent INSERM AMU site

European research network

1. Marie Curie RTN-CT-2005–019335 "Molecular basis of antibiotic translocation", 2005-2008.
2. Chair of COST Action BM0701 "Antibiotic transport and efflux: New strategies to combat bacterial resistance" 2008-2012
3. IMI-ND4BB "Molecular basis of the bacterial cell wall permeability" 2013-2018, chair WP4.
4. ITN-Marie Curie, "Translocation" 2013-2017, chair WP3.

Honors

1. Astra/Zeneca/ESMID Wall of resistance Research Grant 2002 “An analysis of in vivo activity of efflux pump inhibitors in antibiotic-resistant Enterobacter aerogenes”.
2. National French Academy of Medicine, Eloi Collery Price, France 2005, “Bacterial membrane and antibiotic resistance mechanisms.
3. Medal of Honor, Health Department of French Army (SSA-France), 2010.

Present Research Interest

1. Bacterial membrane transporters (genetic, structural and functional aspects, structural-activity relationship),
2. Bacterial adaptation and clinical resistance, mechanisms involved in antibiotic resistance (regulation, activity, etc)
3. Antibiotic resistance mechanisms, mechanism-biochemistry, regulation and expression, activity and clinical involvement
4. New antimicrobial compounds, new antibacterial activity or molecules that target resistance mechanism (inhibitor of efflux pumps)

Publications, Conferences

over 400 scientific publications, abstracts, patents, lectures and communications in antibiotics-bacteriology-biochemistry-chemistry-drugs-membrane domain

Top recent Publications

Vergalli J, Dumont E, Pajović J, Cinquin B, Maigre L, Masi M, Réfrégiers M, Pagès JM. Quantification of antibiotic fluorescence in intact cells for the characterization of translocation across bacterial membranes. Nat Protoc., 2018 13(6):1348-1361.

Davin-Regli A, Lavigne JP, Pagès JM. Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance. Clin Microbiol Rev. 2019 Jul 17;32(4). pii: e00002-19. doi: 10.1128/CMR.00002-19.

Vergalli J, Bodrenko IV, Masi M, Moynié L, Acosta-Gutiérrez S, Naismith JH, Davin-Regli A, Ceccarelli M, van den Berg B, Winterhalter M, Pagès JM. Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria. Nat Rev Microbiol. 2020 Mar;18(3):164-176. doi: 10.1038/s41579-019-0294-2.

Troudi A, Pagès JM, Brunel JM. Chemical Highlights Supporting the Role of Lipid A in Efficient Biological Adaptation of Gram-Negative Bacteria to External Stresses. J Med Chem. 2021 Feb 25;64(4):1816-1834. doi: 10.1021/acs.jmedchem.0c02185. Epub 2021 Feb 4.

Masi M, Vergalli J, Ghai I, Barba-Bon A, Schembri T, Nau WM, Lafitte D, Winterhalter M, Pagès JM. Cephalosporin translocation across enterobacterial OmpF and OmpC channels, a filter across the outer membrane. Commun Biol. 2022 Oct 5;5(1):1059. doi: 10.1038/s42003-022-04035-y.

Vergalli J, Atzori A, Pajovic J, Dumont E, Malloci G, Masi M, Vargiu AV, Winterhalter M, Réfrégiers M, Ruggerone P, Pagès JM. The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux. Commun Biol. 2020 Apr 28;3(1):198. doi: 10.1038/s42003-020-0929-x.

Vergalli J, Chauvet H, Oliva F, Pajović J, Malloci G, Vargiu AV, Réfrégiers M, Ruggerone P, Pagès JM. A framework for dissecting affinities of multidrug efflux transporter AcrB to fluoroquinolones. Commun Biol. 2022 Oct 6;5(1):1062. doi: 10.1038/s42003-022-04024-1.

Ferrand A, Vergalli J, Bosi C, Pantel A, Pagès JM, Davin-Regli A. Contribution of efflux and mutations in fluoroquinolone susceptibility in MDR enterobacterial isolates: a quantitative and molecular study. J Antimicrob Chemother. 2023 Jun 1;78(6):1532-1542. doi: 10.1093/jac/dkad122.

Scientific reports-oinions published by DG SANCO public health (2009-2013)

As SCENIHR member, I coordinated and signed several scientific reports-opinions:

1. Chair and Rapporteur, SCENIHR Opinion on: Assessment of the Antibiotic Resistance Effects of Biocides. Adopted/published 2009.
2. Joint Opinion on antimicrobial resistance (AMR) focused on zoonotic infections. EFSA, the European Centre for Disease Prevention and Control, the European Medicines Agency and the European Commission’s Scientific Committee on Emerging and Newly Identified Health Risks published a joint scientific opinion on antimicrobial resistance focused on infections transmitted to humans from animals and food . EFSA-Q-2008-781. Adopted/published 2009.

Chair and Rapporteur, SCENIHR Opinion on: Research strategy to address the knowledge gaps on the antimicrobial resistance effects of biocides. Adopted/published 2010