Journal Article Epublish 82 citations
PubMed: 29296497 DOI: 10.1364/BOE.8.005688
Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging.
Biomed Opt Express 8(12):5688-5697
Authors: Kyoohyun Kim, Wei Sun Park, Sangchan Na, Sangbum Kim, Taehong Kim, Won Do Heo, YongKeun Park
Affiliations
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea.
- KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, South Korea.
- Current address: Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany.
- Department of Biological Sciences, KAIST, Daejeon 34141, South Korea.
- TomoCube Inc. Daejeon 34051, South Korea.
- Department of Biological Sciences, KAIST, Daejeon 34141, South Korea. Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34141, South Korea.
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea. KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, South Korea. TomoCube Inc. Daejeon 34051, South Korea.
Abstract
Optical diffraction tomography (ODT) provides label-free three-dimensional (3D) refractive index (RI) measurement of biological samples. However, due to the nature of the RI values of biological specimens, ODT has limited access to molecular specific information. Here, we present an optical setup combining ODT with three-channel 3D fluorescence microscopy, to enhance the molecular specificity of the 3D RI measurement. The 3D RI distribution and 3D deconvoluted fluorescence images of HeLa cells and NIH-3T3 cells are measured, and the cross-correlative analysis between RI and fluorescence of live cells are presented.
MeSH
Research Design, Tomography, Nih 3T3 Cells
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