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Journal Article Ppublish 33 citations

PubMed: 34130086 DOI: 10.1016/j.bios.2021.113403

Raman and quantitative phase imaging allow morpho-molecular recognition of malignancy and stages of B-cell acute lymphoblastic leukemia.

Biosens Bioelectron 190:113403

Authors: Santosh Kumar Paidi, Piyush Raj, Rosalie Bordett, Chi Zhang, Sukrut H Karandikar, Rishikesh Pandey, Ishan Barman

Affiliations

  • Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Connecticut Children's Innovation Center, University of Connecticut School of Medicine, Farmington, CT, 06032, USA.
  • Department of Immunology, University of Connecticut School of Medicine, Farmington, CT, 06030, USA.
  • Connecticut Children's Innovation Center, University of Connecticut School of Medicine, Farmington, CT, 06032, USA; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
  • Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD, 21287, USA.

Abstract

Acute lymphoblastic leukemia (ALL) is one of the most common malignancies that account for nearly one-third of all pediatric cancers. The current diagnostic assays are time-consuming, labor-intensive, and require expensive reagents. Here, we report a label-free approach featuring diffraction phase imaging and Raman microscopy that can retrieve both morphological and molecular attributes for label-free optical phenotyping of individual B cells. By investigating leukemia cell lines of early and late stages along with the healthy B cells, we show that phase images can capture subtle morphological differences among the healthy, early, and late stages of leukemic cells. By exploiting its biomolecular specificity, we demonstrate that Raman microscopy is capable of accurately identifying not only different stages of leukemia cells but also individual cell lines at each stage. Overall, our study provides a rationale for employing this hybrid modality to screen leukemia cells using the widefield QPI and using Raman microscopy for accurate differentiation of early and late-stage phenotypes. This contrast-free and rapid diagnostic tool exhibits great promise for clinical diagnosis and staging of leukemia in the near future.

MeSH

Electronics, Medical, Microscopy, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Biotechnology, Cells, Neoplasms, Cell Line, B-Lymphocytes, Exhibitions As Topic, Quantitative Phase Imaging

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