Journal Article Epublish 39 citations
PubMed: 33994544 DOI: 10.1038/s41377-021-00535-4
Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping.
Light Sci Appl 10(1):102
Authors: Moosung Lee, Kyoohyun Kim, Jeonghun Oh, YongKeun Park
Affiliations
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon, 34141, South Korea.
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea.
- Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058, Erlangen, Germany.
- Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea. KAIST Institute for Health Science and Technology, KAIST, Daejeon, 34141, South Korea. Tomocube Inc. Daejeon, 34109, South Korea.
Abstract
A major challenge in three-dimensional (3D) microscopy is to obtain accurate spatial information while simultaneously keeping the microscopic samples in their native states. In conventional 3D microscopy, axial resolution is inferior to spatial resolution due to the inaccessibility to side scattering signals. In this study, we demonstrate the isotropic microtomography of free-floating samples by optically rotating a sample. Contrary to previous approaches using optical tweezers with multiple foci which are only applicable to simple shapes, we exploited 3D structured light traps that can stably rotate freestanding complex-shaped microscopic specimens, and side scattering information is measured at various sample orientations to achieve isotropic resolution. The proposed method yields an isotropic resolution of 230 nm and captures structural details of colloidal multimers and live red blood cells, which are inaccessible using conventional tomographic microscopy. We envision that the proposed approach can be deployed for solving diverse imaging problems that are beyond the examples shown here.
MeSH
Microscopy
Cited by
- Bond-selective intensity diffraction tomography. Nat Commun, 2022
- Optical trapping with holographically structured light for single-cell studies. Biophys Rev (Melville), 2023
- Simulation and Experiment of the Trapping Trajectory for Janus Particles in Linearly Polarized Optical Traps. Micromachines (Basel), 2022
- Time-varying 3D optical torque via a single beam. Nat Commun, 2025
- Three-dimensional isotropic imaging of live suspension cells enabled by droplet microvortices. Proc Natl Acad Sci U S A, 2024
- Multimodal image reconstruction from tomographic diffraction microscopy data. J Microsc, 2022
- Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy. Light Sci Appl, 2022
- AI-driven projection tomography with multicore fibre-optic cell rotation. Nat Commun, 2024
- Real-time complex light field generation through a multi-core fiber with deep learning. Sci Rep, 2022
- Optical single-pixel volumetric imaging by three-dimensional light-field illumination. Proc Natl Acad Sci U S A, 2023
- High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching. Biomed Opt Express, 2023
- Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy. Sensors (Basel), 2024