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Journal Article Epublish 32 citations

PubMed: 34220909 DOI: 10.3389/fpls.2021.687799

Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses.

Front Plant Sci 12:687799

Authors: Lalida Sangpong, Gholamreza Khaksar, Pinnapat Pinsorn, Akira Oikawa, Ryosuke Sasaki, Alexander Erban, Mutsumi Watanabe, Karan Wangpaiboon, Takayuki Tohge, Joachim Kopka, Rainer Hoefgen, Kazuki Saito, Supaart Sirikantaramas

Affiliations

  • Molecular Crop Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
  • Faculty of Agriculture, Yamagata University, Yamagata, Japan.
  • Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
  • Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
  • Plant Secondary Metabolism, Graduate School of Science and Technology, Nara Institute of Science and Technology (NAIST), Nara, Japan.
  • Molecular Sensory Science Center, Chulalongkorn University, Bangkok, Thailand.

Abstract

Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, using capillary electrophoresis- and gas chromatography- time-of-flight mass spectrometry, specifically focusing on taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for the first time. The malate and succinate contents increased, while those of citrate, an abundant organic acid, were unchanged. Notably, most amino acids increased, including isoleucine, leucine, and valine, whereas aspartate decreased, and glutamate was unchanged. Furthermore, transcriptomic analysis was performed to analyze the dynamic changes in sugar metabolism, glycolysis, TCA cycle, and amino acid pathways to identify key candidate genes. Taken together, our results elucidate the fundamental taste-related metabolism of durian, which can be exploited to develop durian metabolic and genetic markers in the future.

MeSH

Fruit, Amino Acids, Taste, Leucine, Gene Expression Profiling, Metabolomics

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