Journal Article Ppublish 14 citations
PubMed: 34819398 DOI: 10.14348/molcells.2021.0190
Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography.
Mol Cells 44(11):851-860
Authors: Youngkyu Kim, Tae-Keun Kim, Yeonhee Shin, Eunyoung Tak, Gi-Won Song, Yeon-Mok Oh, Jun Ki Kim, Chan-Gi Pack
Affiliations
- Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Korea.
- Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea.
- Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Korea. Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea.
- Division of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.
- Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.
Abstract
Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells-namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell-based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.
MeSH
Organelles, Cells, Microscopy, Electron, Scanning Transmission, Molecular Biology, Tomography, Stem Cells
Cited by
- Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy. Mol Cells, 2022
- Enhanced functionalities of immune cells separated by a microfluidic lattice: assessment based on holotomography. Biomed Opt Express, 2024
- Fasudil and viscosity of gelatin promote hepatic differentiation by regulating organelles in human umbilical cord matrix-mesenchymal stem cells. Stem Cell Res Ther, 2024
- Hybrid machine-learning framework for volumetric segmentation and quantification of vacuoles in individual yeast cells using holotomography. Biomed Opt Express, 2023
- Molecular tracking of interactions between progenitor and endothelial cells via Raman and FTIR spectroscopy imaging: a proof of concept of a new analytical strategy for in vitro research. Cell Mol Life Sci, 2023