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Journal Article Epublish 9 citations

PubMed: 35011551 DOI: 10.3390/molecules27010317

Different Fruit-Specific Promoters Drive Expression to Improve Phenylpropanoid Accumulation in Tomato.

Molecules 27(1)

Authors: Xiangyu Ding, Ziyi Yin, Shaoli Wang, Haoqi Liu, Xiaomeng Chu, Jiazong Liu, Haipeng Zhao, Xinyu Wang, Yang Li, Xinhua Ding

Affiliations

  • State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
  • Yantai Academy of Agricultural Sciences, Yantai 265500, China.

Abstract

Tomato is an economically crucial vegetable/fruit crop globally. Tomato is rich in nutrition and plays an essential role in a healthy human diet. Phenylpropanoid, a critical compound in tomatoes, reduces common degenerative and chronic diseases risk caused by oxidative stress. As an MYB transcription factor, can increase phenylpropanoid content by activating phenylpropanoid synthesis related genes, such as PAL, C4H, 4CL, CHS. However, the heterologous expression of in tomatoes can be altered through transgenic technologies, such as unstable expression vectors and promoters with different efficiency. In the current study, the efficiency of other fruit-specific promoters, namely E8S, 2A12, E4, and PG, were compared and screened, and we determined that the expression efficiency of was driven by the E8S promoter was the highest. As a result, the expression of phenylpropanoid synthesis related genes was regulated by , and the phenylpropanoid accumulation in transgenic tomato fruits increased 16 times. Additionally, the total antioxidant capacity of fruits was measured through Trolox equivalent antioxidant capacity (TEAC) assay, which was increased by 2.4 times in E8S transgenic lines. TEAC was positively correlated with phenylpropanoid content. Since phenylpropanoid plays a crucial role in the human diet, expressing with stable and effective fruit-specific promoter E8S could improve tomato's phenylpropanoid and nutrition content and quality. Our results can provide genetic resources for the subsequent improvement of tomato varieties and quality, which is significant for human health.

MeSH

Genes, Flavonols, Promoter Regions, Genetic, Diet, Fruit, Antioxidants

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