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Journal Article Research Support, Non-U.S. Gov't Epublish 10 citations

PubMed: 35563796 DOI: 10.3390/cells11091490

Depletion of γδ T Cells Leads to Reduced Angiogenesis and Increased Infiltration of Inflammatory M1-like Macrophages in Ischemic Muscle Tissue.

Cells 11(9)

Authors: Christoph Arnholdt, Konda Kumaraswami, Philipp Götz, Matthias Kübler, Manuel Lasch, Elisabeth Deindl

Affiliations

  • Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, 81377 Munich, Germany.
  • Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
  • Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig-Maximilians-Universität München, 81377 Munich, Germany.

Abstract

γδ T cells, a small subset of T cells in blood, play a substantial role in influencing immunoregulatory and inflammatory processes. The functional impact of γδ T cells on angiogenesis in ischemic muscle tissue has never been reported and is the topic of the present work. Femoral artery ligation (FAL) was used to induce angiogenesis in the lower leg of γδ T cell depleted mice and wildtype and isotype antibody-treated control groups. Gastrocnemius muscle tissue was harvested 3 and 7 days after FAL and assessed using (immuno-)histological analyses. Hematoxylin and Eosin staining showed an increased area of tissue damage in γδ T cell depleted mice 7 days after FAL. Impaired angiogenesis was demonstrated by lower capillary to muscle fiber ratio and decreased number of proliferating endothelial cells (CD31/BrdU). γδ T cell depleted mice showed an increased number of total leukocytes (CD45), neutrophils (MPO) and neutrophil extracellular traps (NETs) (MPO/CitH3), without changes in the neutrophils to NETs ratio. Moreover, the depletion resulted in a higher macrophage count (DAPI/CD68) caused by an increase in inflammatory M1-like macrophages (CD68/MRC1). Altogether, we show that depletion of γδ T cells leads to increased accumulation of leukocytes and M1-like macrophages, along with impaired angiogenesis.

MeSH

Ischemia, Macrophages, Cells, T-Lymphocytes, Neutrophils, Leukocytes, Tissues, Muscles, Angiogenesis

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