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Journal Article Epublish 87 citations

PubMed: 36412883 DOI: 10.3390/jfb13040242

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Causing Aspergillosis: Ultrastructure Study.

J Funct Biomater 13(4)

Authors: Amr H Hashem, Ebrahim Saied, Basma H Amin, Fatimah O Alotibi, Abdulaziz A Al-Askar, Amr A Arishi, Fathy M Elkady, Mostafa A Elbahnasawy

Affiliations

  • Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo 11884, Egypt.
  • Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia.
  • School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia.
  • Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt.

Abstract

Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common , including , , , and at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against , , , and were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall's integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of can be used as a promising antifungal agent against species causing Aspergillosis.

MeSH

Cell Wall, Cells, Nanoparticles, Aspergillosis

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