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Publications

Journal Article Review Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Ppublish 38 citations

PubMed: 37248392 DOI: 10.1038/s41596-023-00829-4

Spatial- and Fourier-domain ptychography for high-throughput bio-imaging.

Nat Protoc 18(7):2051-2083

Authors: Shaowei Jiang, Pengming Song, Tianbo Wang, Liming Yang, Ruihai Wang, Chengfei Guo, Bin Feng, Andrew Maiden, Guoan Zheng

Affiliations

  • Department of Biomedical Engineering, University of Connecticut, Storrs, USA.
  • Hangzhou Institute of Technology, Xidian University, Hangzhou, China.
  • Department of Electronic and Electrical Engineering, University of Sheffield, Sheffield, UK.
  • Diamond Light Source, Harwell Science and Innovation Campus, Chilton, UK.
  • Department of Biomedical Engineering, University of Connecticut, Storrs, USA.

Abstract

First envisioned for determining crystalline structures, ptychography has become a useful imaging tool for microscopists. However, ptychography remains underused by biomedical researchers due to its limited resolution and throughput in the visible light regime. Recent developments of spatial- and Fourier-domain ptychography have successfully addressed these issues and now offer the potential for high-resolution, high-throughput optical imaging with minimal hardware modifications to existing microscopy setups, often providing an excellent trade-off between resolution and field of view inherent to conventional imaging systems, giving biomedical researchers the best of both worlds. Here, we provide extensive information to enable the implementation of ptychography by biomedical researchers in the visible light regime. We first discuss the intrinsic connections between spatial-domain coded ptychography and Fourier ptychography. A step-by-step guide then provides the user instructions for developing both systems with practical examples. In the spatial-domain implementation, we explain how a large-scale, high-performance blood-cell lens can be made at negligible expense. In the Fourier-domain implementation, we explain how adding a low-cost light source to a regular microscope can improve the resolution beyond the limit of the objective lens. The turnkey operation of these setups is suitable for use by professional research laboratories, as well as citizen scientists. Users with basic experience in optics and programming can build the setups within a week. The do-it-yourself nature of the setups also allows these procedures to be implemented in laboratory courses related to Fourier optics, biomedical instrumentation, digital image processing, robotics and capstone projects.

MeSH

Cells, Laboratories, Clinical Laboratory Techniques, Research

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