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Journal Article Ppublish 31 citations

PubMed: 38231557 DOI: 10.1128/mbio.02958-23

PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge.

mBio 15(2):e0295823

Authors: Yongxiang Zhao, Baochao Fan, Xu Song, Jie Gao, Rongli Guo, Cheng Yi, Zhaoming He, Hongpeng Hu, Jianhao Jiang, Lixiang Zhao, Tianyi Zhong, Bin Li

Affiliations

  • Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. GuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, China.
  • Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
  • Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China.
  • Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
  • Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
  • Suzhou Huiliao Biomedical Technology Co. Ltd. Suzhou, China.
  • Suzhou Huiliao Biomedical Technology Co. Ltd. Suzhou, China. Suzhou Medical College, Soochow University, Suzhou, China.

Abstract

Porcine epidemic diarrhea virus (PEDV), a swine enteropathogenic coronavirus, causes severe diarrhea in neonatal piglets, which is associated with a high mortality rate. Thus, developing effective and safe vaccines remains a top priority for controlling PEDV infection. Here, we designed two lipid nanoparticle (LNP)-encapsulated mRNA (mRNA-LNP) vaccines encoding either the full-length PEDV spike (S) protein or a multiepitope chimeric spike (Sm) protein. We found that the S mRNA-LNP vaccine was superior to the Sm mRNA-LNP vaccine at inducing antibody and cellular immune responses in mice. Evaluation of the immunogenicity and efficacy of the S mRNA vaccine in piglets confirmed that it induced robust PEDV-specific humoral and cellular immune responses . Importantly, the S mRNA-LNP vaccine not only protected actively immunized piglets against PEDV but also equipped neonatal piglets with effective passive anti-PEDV immunity in the form of colostrum-derived antibodies after the immunization of sows. Our findings suggest that the PEDV-S mRNA-LNP vaccine is a promising candidate for combating PEDV infection.IMPORTANCEPorcine epidemic diarrhea virus (PEDV) continues to harm the global swine industry. It is important to develop a highly effective vaccine to control PEDV infection. Here, we report a PEDV spike (S) mRNA vaccine that primes a potent antibody response and antigen-specific T-cell responses in immunized piglets. Active and passive immunization can protect piglets against PED following the virus challenge. This study highlights the efficiency of the PEDV-S mRNA vaccine and represents a viable approach for developing an efficient PEDV vaccine.

MeSH

Cells, Immunization, Vaccines, Rna, Messenger, Microbiological Phenomena, Viruses, Immunity, Diarrhea, Epidemics, Infections, Lipid Nanoparticles

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