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Publications

Journal Article Review Research Support, Non-U.S. Gov't Epublish 4 citations

PubMed: 38706963 DOI: 10.1093/function/zqae012

Systems Approaches to Cell Culture-Derived Extracellular Vesicles for Acute Kidney Injury Therapy: Prospects and Challenges.

Function (Oxf) 5(3):zqae012

Authors: David J Lundy, Barbara Szomolay, Chia-Te Liao

Affiliations

  • Graduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Taipei 235603, Taiwan.
  • International PhD Program in Biomedical Engineering, Taipei Medical University, Taipei 235603, Taiwan.
  • Center for Cell Therapy, Taipei Medical University Hospital, Taipei 110301, Taiwan.
  • Systems Immunity Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.
  • Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.
  • Division of Nephrology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan.
  • Division of Nephrology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
  • Research Center of Urology and Kidney, Taipei Medical University, Taipei 110, Taiwan.

Abstract

Acute kidney injury (AKI) is a heterogeneous syndrome, comprising diverse etiologies of kidney insults that result in high mortality and morbidity if not well managed. Although great efforts have been made to investigate underlying pathogenic mechanisms of AKI, there are limited therapeutic strategies available. Extracellular vesicles (EV) are membrane-bound vesicles secreted by various cell types, which can serve as cell-free therapy through transfer of bioactive molecules. In this review, we first overview the AKI syndrome and EV biology, with a particular focus on the technical aspects and therapeutic application of cell culture-derived EVs. Second, we illustrate how multi-omic approaches to EV miRNA, protein, and genomic cargo analysis can yield new insights into their mechanisms of action and address unresolved questions in the field. We then summarize major experimental evidence regarding the therapeutic potential of EVs in AKI, which we subdivide into stem cell and non-stem cell-derived EVs. Finally, we highlight the challenges and opportunities related to the clinical translation of animal studies into human patients.

MeSH

Physiological Phenomena, Stem Cells, Therapeutics, Microscopy, Electron, Scanning Transmission, Cell Culture Techniques, Systems Biology, Acute Kidney Injury, Extracellular Vesicles

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