Journal Article Research Support, Non-U.S. Gov't Ppublish 3 citations
PubMed: 38743977 DOI: 10.1016/j.jhazmat.2024.134562
Biocompatible plasma-treated liquids: A sustainable approach for decontaminating gastrointestinal-infection causing pathogens.
J Hazard Mater 472:134562
Authors: Manorma Negi, Neha Kaushik, Prajwal Lamichhane, Apurva Jaiswal, Shweta B Borkar, Paritosh Patel, Prashant Singh, Eun Ha Choi, Nagendra Kumar Kaushik
Affiliations
- Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, South Korea.
- Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, South Korea.
- Department of Chemistry, Atma Ram Sanatan Dharma College, University of Delhi, Delhi, India.
- Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, South Korea.
- Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, South Korea.
Abstract
Nosocomial infections are a serious threat and difficult to cure due to rising antibiotic resistance in pathogens and biofilms. Direct exposure to cold atmospheric plasma (CAP) has been widely employed in numerous biological research endeavors. Nonetheless, plasma-treated liquids (PTLs) formulated with physiological solutions may offer additional benefits such as enhanced portability, and biocompatibility. Additionally, CAP-infused long-lived reactive oxygen and nitrogen species (RONS) such as nitrite (NO), nitrate (NO), and hydrogen peroxide (HO) can synergistically induce their antibacterial activity. Herein, we investigated those argon-plasma jet-treated liquids, including Ringer's lactate (RL), phosphate-buffered saline (PBS), and physiological saline, have significant antibacterial activity against nosocomial/gastrointestinal-causing pathogens, which might be due to ROS-mediated lipid peroxidation. Combining the conventional culture-based method with propidium iodide monoazide quantitative PCR (PMAxxâ„¢-qPCR) indicated that PTLs induce a minimal viable but non-culturable (VBNC) state and moderately affect culturable counts. Specifically, the PTL exposure resulted in pathogenicity dysfunction via controlling T3SS-related effector genes of S. enterica. Overall, this study provides insights into the effectiveness of PTLs for inducing ROS-mediated damage, controlling the virulence of diarrheagenic bacteria, and modulating homeostatic genes.
MeSH
Genes, Bacteria, Infections, Hazardous Substances, Plasma