Journal Article Ppublish 1 citation
PubMed: 40099802 DOI: 10.1021/acs.orglett.5c00284
Introducing Rigidity into the GFP Chromophore via a Boron Bridge: Insights and Application in Two-Photon Imaging.
Org Lett 27(13):3128-3133
Authors: Attila Csomos, Brigitta Petrilla, Levente Cseri, Gábor Turczel, Arnold Steckel, Anett Matuscsák, Gitta Schlosser, Balázs J Rózsa, Ervin Kovács, Zoltán Mucsi
Affiliations
- Femtonics Ltd. H-1087 Budapest, Hungary.
- ELTE Hevesy György PhD School of Chemistry, H-1117 Budapest, Hungary.
- BrainVisionCenter, H-1094 Budapest, Hungary.
- NMR Research Laboratory, Centre for Structural Science, HUN-REN Research Centre for Natural Sciences, H-1117 Budapest, Hungary.
- MTA-ELTE Lendület (Momentum) Ion Mobility Mass Spectrometry Research Group, Faculty of Science, Institute of Chemistry, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, H-1117 Budapest, Hungary.
- The Faculty of Information Technology, Pázmány Péter Catholic University, H-1083 Budapest, Hungary.
- Laboratory of 3D Functional Network and Dendritic Imaging, HUN-REN Institute of Experimental Medicine, H-1083 Budapest, Hungary.
- Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, H-1117 Budapest, Hungary.
- University of Miskolc, H-3515 Miskolc, Hungary.
Abstract
A new family of azaborine fluorophores, consisting of ten compounds, has been developed that mimics the chromophore of green fluorescent protein, with conformation locked by a hydroxyboron bridge. These fluorophores exhibit fluorescence Stokes shifts up to 100 nm (0.56 eV) and high brightness (>10 M cm) in the 408-525 nm range. Their potential utility for sensing applications was demonstrated by constructing zinc sensors capable of detecting 100 micromolar Zn concentrations in biological systems with two-photon microscopy.
MeSH
Chemistry, Organic