Journal Article Epublish
PubMed: 41972690 DOI: 10.3390/cells15070600
Static Magnetic Field-Mediated Parathyroid Xenotransplantation Modulates Lymphocyte Migration: A Potential Immunosuppression-Free Long-Term Treatment for Hypoparathyroidism.
Cells 15(7)
Authors: Ahmed Alperen Tuncer, Gülnihal Bozdağ, Ezgi Hacıhasanoğlu, Özge Karabıyık Acar, Fikrettin Şahin, Gamze Torun Köse, Erhan Ayşan
Affiliations
- Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul 34755, Türkiye.
- Department of Pathology, Istanbul Training and Research Hospital, Istanbul 34098, Türkiye.
- Department of Genetics and Bioengineering, Istanbul Okan University, Istanbul 34959, Türkiye.
- Department of General Surgery, Yeditepe University Hospital, Istanbul 34718, Türkiye.
Abstract
Static magnetic fields (SMFs) are underexplored as biophysical tools for transplant immunomodulation. This study investigated a 300 mT SMF as a non-pharmacological adjuvant to enhance graft survival in parathyroid xenotransplantation. Human parathyroid tissues were transplanted into Sprague-Dawley rats (n = 20) across four groups: control (G1), SMF-only (G2), transplantation-only (G3), and SMF-assisted transplantation (G4). Following 30-day continuous SMF exposure, functional and immunological assessments were performed. G4 achieved the highest systemic PTH recovery (p = 0.009) without altering intrinsic secretory capacity. Systemic cytokine profiling revealed significant IFN-gamma suppression in G4 (p = 0.0024), suggesting downregulation of Th1-mediated rejection pathways. While G2 showed pro-inflammatory increases (TNF-alpha, GM-CSF), G4 maintained baseline levels, confirming biocompatibility. IHC confirmed that SMF exposure sequestered lymphocytes to the graft periphery, preventing the diffuse infiltration observed in G3. In conclusion, continuous SMF exposure modulates the immune microenvironment by altering lymphocyte migration and IFN-gamma signaling. This biophysical strategy provides localized immunoprotection, potentially offering a drug-free alternative to systemic immunosuppression in endocrine tissue transplantation.
MeSH
Parathyroid Hormone, Cells, Transplantation, Heterologous, Hypoparathyroidism, Lymphocytes, Immunity, Transplants, Therapeutics, Tissues, Immunosuppression Therapy, Transplantation