System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Epublish

PubMed: 42222785 DOI: 10.1021/acsomega.5c11436

Biogenic Silica Nanoparticles Enhance Beauveria bassiana Bioavailability and Efficacy for Sustainable Pest Control.

ACS Omega 11(20):29525-29540

Authors: Liliam Katsue Harada, Mariana Guilger-Casagrande, Tais Germano-Costa, Natália Bilesky-José, Ricardo Antonio Polanczyk, Kelly Cristina Gonçalves, Paulo Gonçalves da Silva, João Vitor Silva E Silva, Renato de Mello Prado, Leonardo F Fraceto, Renata Lima

Affiliations

  • Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO), Rod. Raposo Tavares km 92.5, 18023-000 Sorocaba, São Paulo, Brazil.
  • Laboratory of Microbial Control of Pests, São Paulo State University (UNESP), Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, São Paulo, Brazil.
  • Laboratory of Plant Nutrition, Department of Agricultural Production Sciences (Soil and Fertilizer Sector), São Paulo State University (UNESP), Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, São Paulo, Brazil.
  • Laboratory of Environmental Nanotechnology, São Paulo State University (UNESP), Av. Três de Março 511, 18087-180 Sorocaba, São Paulo, Brazil.

Abstract

Biogenic silicon dioxide nanoparticles (SiO 2-NPs_bio) synthesized using Fusarium oxysporum were combined with Beauveria bassiana in a Pickering emulsion to enhance fungal bioavailability and insecticidal efficacy. The biogenic nanoparticles exhibited larger diameters (324 ± 3 nm) and lower ζ-potential (-23 ± 0.2 mV) than commercial SiO 2-NPs, while showing no significant cytotoxicity in HaCat and 3T3 cell lines. Encapsulation markedly improved fungal performance, resulting in 100% mortality of Spodoptera frugiperda, S. cosmioides, and Chrysodeixis includens, and 86% mortality of Rachiplusia nu, significantly higher than isolated nanoparticles or fungus. The nanoformulation also protected B. bassiana against UV radiation and showed no phytotoxic effects. This study demonstrates, for the first time, a sustainable biogenic SiO 2-based Pickering emulsion as an effective carrier for entomopathogenic fungi in pest control.

MeSH

Pest Control, Beauveria, Mortality, Biological Availability, Nanoparticles, Fungi, Emulsions