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Publications

Journal Article Ahead of print 1 citation

PubMed: 42608461 DOI: 10.1038/s41592-026-03200-z

PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology.

Nat Methods

Authors: Hendrik A Michel, Paige McCallum, Wenrui Wu, Huaying Qiu, Jia Le Lee, Shuli Luo, Chi Ngai Chan, Johanna Schaffenrath, Yao Yu Yeo, Stephanie Pei Tung Yiu, Yang Wang, Lindsay Parmelee, Hongbo Wang, Tania Pannellini, Melinda Burgess, Nourhan El Ahmar, Zoe Xiaozhu Zhang, Colm Keane, Tony Kiat Hon Lim, Sabina Signoretti, Sonia Victoria Del Rincon, Bo Zhao, David R McIlwain, Yunhao Bai, Fei Chen, Roberto Chiarle, Sizun Jiang

Affiliations

  • Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA, USA.
  • Division of Clinical and Translational Research, McGill University, Montréal, Quebec, Canada.
  • Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • Division of Hematopathology, IEO European Institute of Oncology IRCCS, Milan, Italy.
  • Frazer Institute, Translational Research Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
  • Department of Anatomical Pathology, Singapore General Hospital, Singapore, Singapore.
  • Department of Microbiology and Immunology, University of Nevada Reno, Reno, NV, USA.
  • Broad Institute of Harvard and MIT, Cambridge, MA, USA.
  • Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
  • Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
  • Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA, USA.
  • Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
  • Broad Institute of Harvard and MIT, Cambridge, MA, USA.

Abstract

Spatial proteomics is limited by detection sensitivity, multiplexing and multimodal integration, leaving a gap between discovery and clinical assays. Here we present protein and nucleic acid serial tyramide amplification (PASTA), using horseradish peroxidase-mediated oligonucleotide deposition and cyclical imaging for high-plex, multimodal spatial profiling. Compatible with conjugated antibodies and in situ hybridization, PASTA enables simultaneous protein and RNA codetection from formalin-fixed, paraffin-embedded samples, providing a cost-effective bridge from discovery to clinical validation.

MeSH

Oligonucleotides