System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Epublish 59 citations

PubMed: 24223994 DOI: 10.1371/journal.pone.0079680

Decreased hematocrit-to-viscosity ratio and increased lactate dehydrogenase level in patients with sickle cell anemia and recurrent leg ulcers.

PLoS One 8(11):e79680

Authors: Philippe Connes, Yann Lamarre, Marie-Dominique Hardy-Dessources, Nathalie Lemonne, Xavier Waltz, Danièle Mougenel, Martin Mukisi-Mukaza, Marie-Laure Lalanne-Mistrih, Vanessa Tarer, Benoit Tressières, Maryse Etienne-Julan, Marc Romana

Affiliations

  • UMR Inserm 665, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe ; Laboratory of Excellence GR-Ex « The red cell : from genesis to death », PRES Sorbonne Paris Cité, Paris, France ; Laboratoire ACTES (EA 3596)/Département de Physiologie, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.

Abstract

Leg ulcer is a disabling complication in patients with sickle cell anemia (SCA) but the exact pathophysiological mechanisms are unknown. The aim of this study was to identify the hematological and hemorheological alterations associated with recurrent leg ulcers. Sixty-two SCA patients who never experienced leg ulcers (ULC-) and 13 SCA patients with a positive history of recurrent leg ulcers (ULC+)--with no leg ulcers at the time of the study--were recruited. All patients were in steady state condition. Blood was sampled to perform hematological, biochemical (hemolytic markers) and hemorheological analyses (blood viscosity, red blood cell deformability and aggregation properties). The hematocrit-to-viscosity ratio (HVR), which reflects the red blood cell oxygen transport efficiency, was calculated for each subject. Patients from the ULC+ group were older than patients from the ULC- group. Anemia (red blood cell count, hematocrit and hemoglobin levels) was more pronounced in the ULC+ group. Lactate dehydrogenase level was higher in the ULC+ group than in the ULC- group. Neither blood viscosity, nor RBC aggregation properties differed between the two groups. HVR was lower and RBC deformability tended to be reduced in the ULC+ group. Our study confirmed increased hemolytic rate and anemia in SCA patients with leg ulcers recurrence. Furthermore, our data suggest that although systemic blood viscosity is not a major factor involved in the pathophysiology of this complication, decreased red blood cell oxygen transport efficiency (i.e., low hematocrit/viscosity ratio) may play a role.

MeSH

Erythrocytes, Anemia, Sickle Cell, Oxidoreductases, Blood Cells, Leg Ulcer, Hematocrit, Blood Viscosity, L-Lactate Dehydrogenase

Cited by