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Journal Article Epublish 26 citations

PubMed: 35574449 DOI: 10.3389/fphys.2022.884690

Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications.

Front Physiol 13:884690

Authors: Steffen M Recktenwald, Marcelle G M Lopes, Stephana Peter, Sebastian Hof, Greta Simionato, Kevin Peikert, Andreas Hermann, Adrian Danek, Kai van Bentum, Hermann Eichler, Christian Wagner, Stephan Quint, Lars Kaestner

Affiliations

  • Experimental Physics, Saarland University, Saarbruecken, Germany.
  • Cysmic GmbH, Saarbruecken, Germany.
  • Experimental Physics, Saarland University, Saarbruecken, Germany. Theoretical Medicine and Biosciences, Saarland University, Saarbruecken, Germany.
  • Institute for Clinical and Experimental Surgery, Saarland University, Campus University Hospital, Homburg, Germany.
  • Translational Neurodegeneration Section "Albrecht-Kossel", Department of Neurology, University Medical Center Rostock, University of Rostock, Rostock, Germany.
  • DZNE, Deutsches Zentrum für Neurodegenerative Erkrankungen, Research Site Rostock/Greifswald, Rostock, Germany.
  • Center for Transdisciplinary Neurosciences Rostock (CTNR), University Medical Center Rostock, University of Rostock, Rostock, Germany.
  • Neurologische Klinik und Poliklinik, Ludwig-Maximilians-University, Munich, Germany.
  • MVZ Saarpfalz, Homburg, Germany.
  • Institute for Clinical Hemostaseology and Transfusion Medicine, Saarland University and Saarland University Hospital, Homburg, Germany.
  • Department of Physics and Materials Science, University of Luxembourg, Luxembourg City, Luxembourg.
  • Theoretical Medicine and Biosciences, Saarland University, Saarbruecken, Germany.

Abstract

In many medical disciplines, red blood cells are discovered to be biomarkers since they "experience" various conditions in basically all organs of the body. Classical examples are diabetes and hypercholesterolemia. However, recently the red blood cell distribution width (RDW), is often referred to, as an unspecific parameter/marker (e.g., for cardiac events or in oncological studies). The measurement of RDW requires venous blood samples to perform the complete blood cell count (CBC). Here, we introduce Erysense, a lab-on-a-chip-based point-of-care device, to evaluate red blood cell flow properties. The capillary chip technology in combination with algorithms based on artificial neural networks allows the detection of very subtle changes in the red blood cell morphology. This flow-based method closely resembles conditions and blood sample volumes in the sub-microliter range are sufficient. We provide clinical examples for potential applications of Erysense as a diagnostic tool [here: neuroacanthocytosis syndromes (NAS)] and as cellular quality control for red blood cells [here: hemodiafiltration (HDF) and erythrocyte concentrate (EC) storage]. Due to the wide range of the applicable flow velocities (0.1-10 mm/s) different mechanical properties of the red blood cells can be addressed with Erysense providing the opportunity for differential diagnosis/judgments. Due to these versatile properties, we anticipate the value of Erysense for further diagnostic, prognostic, and theragnostic applications including but not limited to diabetes, iron deficiency, COVID-19, rheumatism, various red blood cell disorders and anemia, as well as inflammation-based diseases including sepsis.

MeSH

Diabetes Mellitus, Cells, Erythrocytes, Blood, Point-Of-Care Systems, Microfluidics, Blood Cells, Hemodiafiltration

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