Journal Article Journal Article Epublish 1 citation
PubMed: 39695163 DOI: 10.1038/s41597-024-04235-7
A new chromosome-level genome assembly and annotation of Cryptosporidium meleagridis.
Sci Data 11(1):1388
Authors: Lasya R Penumarthi, Rodrigo P Baptista, Megan S Beaudry, Travis C Glenn, Jessica C Kissinger
Affiliations
- Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA. Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
- Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA.
- Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
- Department of Medicine, Weill Cornell Medical College, New York City, NY, 10065, USA.
- Division of Infectious Diseases, Houston Methodist Research Institute, Houston, TX, 77030, USA.
- Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA. Genomics, Daicel Arbor Biosciences, Ann Arbor, MI, 48103, USA.
- Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA.
- Department of Genetics, University of Georgia, Athens, GA, 30602, USA.
- Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA.
- Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
- Division of Infectious Diseases, Houston Methodist Research Institute, Houston, TX, 77030, USA.
Abstract
Cryptosporidium spp. are medically and scientifically relevant protozoan parasites that cause severe diarrheal illness in infants, immunosuppressed populations and many animals. Although most human Cryptosporidium infections are caused by C. parvum and C. hominis, there are several other human-infecting species including C. meleagridis, which are commonly observed in developing countries. Here, we annotated a hybrid long-read Oxford Nanopore Technologies and short-read Illumina genome assembly for C. meleagridis (CmTU1867) with DNA generated using multiple displacement amplification. The assembly was then compared to the previous C. meleagridis (CmUKMEL1) assembly and annotation and a recent telomere-to-telomere C. parvum genome assembly. The chromosome-level assembly is 9.2 Mb with a contig N50 of 1.1 Mb. Annotation revealed 3,919 protein-encoding genes. A BUSCO analysis indicates a completeness of 96.6%. The new annotation contains 166 additional protein-encoding genes and reveals high synteny to C. parvum IOWA II (CpBGF). The new C. meleagridis genome assembly is nearly gap-free and provides a valuable new resource for the Cryptosporidium community and future studies on evolution and host-specificity.
MeSH
Genes, Genome, Chromosomes, Telomere